The High-Throughput Genomics (HTG) Shared Resource provides experimental support for diverse genomic applications that utilize the Illumina sequencing platform. The Resource operates as a full-service facility that offers expertise in all aspects of the experimental process including evaluation of sample quality, construction of Illumina sequencing libraries (genomic DNA, exome and targeted enrichment, methylation analysis, ChIP-seq, RNA-Seq, Small RNA-Seq, ribosome profiling, and single cell analysis), qualifying and normalizing libraries and sequencing on an Illumina instrument.
Key instrumentation within the 1800 ft2 laboratory includes an Illumina NovaSeq 6000, two Illumina HiSeq 2500 instruments, two MiSeq instruments, a 10X Genomics Chromium Controller, a Fluidigm C1 Single-Cell AutoPrep System, an Agilent 2200 TapeStation, a Covaris E210 and an S2 Focussed-Ultrasonnicator, and a Bio-Rad CFX Connect Real Time System. A database and LIMS system, GNomEx, has been designed and implemented to track and store experimental information and enable data distribution.
